RSM logo
Annals of Clinical Biochemistry

Home Current issue Browse archive Alerts About the journal Feedback
 
Ann Clin Biochem 2000;37:319-325
doi:10.1258/0004563001899212
© 2000 Association for Clinical Biochemistry

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ayden, K. H
Right arrow Articles by Bundred, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Original Articles

Radioimmunoassay for the measurement of thrombospondin in plasma and breast cyst fluid: validation and clinical application

Katharine H Ayden, Lesley Tetlow, Ged Byrne and Nigel Bundred

Thrombospondin is an adhesive protein that has been implicated in malignancy, specifically in tumour progression and angiogenesis. We developed a radioimmunoassay for the measurement of thrombospondin in plasma and breast cyst fluid. The assay exhibited high accuracy, with recoveries of 102-136% and acceptable imprecision, with an intra-assay coefficient of variation (CV) of < 7·5% across the analytical range 30-1000 ng/mL and inter-assay CV of 4·4% and 7·7% at 152 and 224 ng/mL, respectively. Thrombospondin measured in the breast cyst fluid of patients with gross cystic disease of the breast showed that patients with type II (Na+) cysts had significantly higher concentrations than type I (K+) cysts. The plasma thrombospondin reference range was determined as 131-274 ng/mL. Patients with breast cancer had significantly higher plasma thrombospondin concentrations than normal individuals or patients with benign breast disease. Plasma thrombospondin was higher in breast cancer patients with lymph node involvement.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Physiol.Home page
L. Anderson
Candidate-based proteomics in the search for biomarkers of cardiovascular disease
J. Physiol., February 15, 2005; 563(1): 23 - 60.
[Abstract] [Full Text] [PDF]



  RSM Press eJournals Collection 2010