Annals of Clinical Biochemistry > Volume 45, Number 1 > Pp. 91-92

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Short Report

Extent of bilirubin interference with Beckman creatinine methods

Goce Dimeski , Brett McWhinney, Brock Jones, Rebecca Mason and Andrew Carter

Department of Chemical Pathology, Queensland Health Pathology Service (QHPS), Princess Alexandra Hospital, Ipswich Road, Woolloongabba, Queensland 4102, Australia

Corresponding author: Goce Dimeski. Email: Goce_Dimeski{at}health.qld.gov.au

Background: The aim of the study was to determine the extent of bilirubin interference on two different Beckman–Coulter creatinine methods used on the CX5 PRO/DxC 600 and the DxC 800 systems, respectively.

Methods: The Beckman methods were compared with a high-performance liquid chromatography (HPLC) method using 104 patient samples with creatinine concentrations <133 µmol/L and bilirubin concentrations of between 30 and 802 µmol/L.

Results: The linear regression analysis calculations expressing the relationship between bilirubin concentration and the observed difference between the Beckman and HPLC creatinine methods are: DxC800 creatinine M (modular or cup) method: r² 0.217, slope –0.023 and intercept 6.43; and DxC600 method: r² 0.7324, slope –0.0882 and intercept 12.01.

Conclusions: There is significantly greater interference by bilirubin on the CX5 PRO/DxC 600 method, which can lead to inaccuracy in the calculation of the estimated glomerular filtration rate by the Modification of Diet in Renal Disease equation.

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