RSM logo
Annals of Clinical Biochemistry

Home Current issue Browse archive Alerts About the journal Feedback
 
Ann Clin Biochem 2008;45:364-368
doi:10.1258/acb.2008.007242
© 2008 Association for Clinical Biochemistry

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via ISI Web of Science (1)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hosogaya, S.
Right arrow Articles by Ozaki, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Original Articles

Measurement of plasma lysophosphatidic acid concentration in healthy subjects: strong correlation with lysophospholipase D activity

Shigemi Hosogaya1, Yutaka Yatomi2,3, Kazuhiro Nakamura2, Ryunosuke Ohkawa2, Shigeo Okubo2, Hiromitsu Yokota2, Masato Ohta1, Hirokazu Yamazaki1, Toru Koike1 and Yukio Ozaki1


1 Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Yamanashi; 2 Department of Clinical Laboratory, The University of Tokyo Hospital; 3 Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan


Corresponding author: Dr Yutaka Yatomi. Email: yatoyuta-tky{at}umin.ac.jp

Background: Lysophosphatidic acid (LPA) plays important roles in a variety of biological responses, especially in the area of vascular biology, and the determination of its plasma concentration is believed to be important. Several mechanisms are known to be involved in the metabolism of LPA.

Methods: To identify factors that may determine the plasma concentrations of this important bioactive lipid, we examined its concentrations using an enzymatic cycling assay and related parameters in 146 healthy subjects.

Results: The LPA concentration was significantly higher in women (mean ± SD, 0.103 ± 0.032 µmol/L; n = 47) than in men (0.077 ± 0.026 µmol/L; n = 99). A multiple regression analysis showed a strong positive correlation between the plasma LPA concentration and serum lysophospholipase D (lysoPLD) activity, while the LPA concentration was correlated with the plasma lysophosphatidylcholine (LPC) concentration only in men. Other lipid-related parameters were only slightly correlated or were not correlated with the LPA concentration.

Conclusions: Our findings suggested that conversion from LPC by lysoPLD might be the major route for LPA production in plasma.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




Acute CT - A Primer of Emergency Imaging

 Harley Street - A brief history Frameworks for Radiology Reporting