Annals of Clinical Biochemistry > Volume 45, Number 4 > Pp. 413-417

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Tabushi, Y. Articles by Takubo, T. Search for Related Content PubMed PubMed Citation Social Bookmarking                      What's this?

Original Articles

Detection of citrullinated proteins in synovial fluids derived from patients with rheumatoid arthritis by proteomics-based analysis

Yoko Tabushi¹, Toyofumi Nakanishi¹, Tohru Takeuchi², Mikio Nakajima³, Kazuhito Ueda⁴, Takuya Kotani², Shigeki Makino², Akira Shimizu¹, Toshiaki Hanafusa² and Takayuki Takubo¹

¹ Department of Clinical Pathology; ² First Department of Internal Medicine; ³ Department of Orthopedic Surgery; ⁴ Central Clinical Laboratory, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki-city, Osaka, 569-8686, Japan

Corresponding author: Dr Tohru Takeuchi. Email: t-takeuchi{at}poh.osaka-med.ac.jp

Background: Rheumatoid arthritis (RA) is the most common inflammatory joint disease. The aetiology of RA remains unknown, but autoimmune responses are considered to play an important role in the disease pathophysiology. Currently available data suggests that the process of diagnosing RA may benefit from testing for anticyclic citrullinated peptides. Identification of the presence of citrullinated proteins in rheumatoid synovial fluids is important for the elucidation of the aetiology of RA as well as in the differential diagnosis of rheumatic-related diseases.

Methods: A proteomics-based approach using electrophoresis/mass spectrometry was applied to identify the citrullinated proteins in synovial fluids from patients with RA. Synovial fluids from patients with RA were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western blot analysis to detect the citrullinated proteins. Identification bands were then subjected to mass spectrometry.

Results: Three proteins – citrullinated fibrinogen, citrullinated fibronectin and citrullinated vimentin – in synovial fluids from RA patients were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

Conclusions: Proteomics-based analysis can be used to detect citrullinated proteins in synovial fluids from RA patients.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?