Annals of Clinical Biochemistry > Volume 46, Number 2 > Pp. 114-116

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Original Articles

Evaluation of the particle gel diffusion technique for the detection of haemoglobin S in Sudanese patients

Ahmed A Nimir¹, Eltahir A G Khalil², Ahmed M Musa², Habab M Yassin^1,3 and Ahmed M El-Hassan¹

¹ Faculty of Laboratory Medicine; ² Institute of Endemic Diseases, University of Khartoum; ³ Faculty of Science, Alneilain University, Khartoum, Sudan

Corresponding author: Prof Eltahir Awad Gasim Khalil, Institute of Endemic Diseases, University of Khartoum, Khartoum P.O. Box 45235, Sudan. Email: eltahirk{at}iend.org, eltahirgasim{at}yahoo.ca

Background: Sickle cell disease is a heterogenous disorder characterized by an abnormal haemoglobin and sickling phenomena of red cells. It is prevalent among certain nomadic tribes in Sudan. Painful, aplastic and haemolytic crises mark sickle cell anaemia. Haemoglobin S (HbS) is detected using haemoglobin electrophoresis, iso-electric focusing and/or high-performance liquid chromatography techniques with high sensitivity, but entails cost and expertise. This study aimed to determine the sensitivity, specificity and positive predictive values (PPV) of the ID-particle gel diffusion technique for screening, diagnosis and phenotyping of HbS in patients with a provisional diagnosis of abnormal haemoglobin.

Methods: Following informed consent, 100 sequential individuals who reported to a central referral haemoglobinopathy clinic were enrolled. ID-particle gel diffusion technique was compared with cellulose acetate electrophoresis to determine haemoglobin phenotypes.

Results: The ID-particle gel test detected HbAA with 100% sensitivity and 100% specificity. Sensitivity for HbS was 100%, whether as HbSS or as a mixed pattern. HbSS was identified in all cases where this is the only haemoglobin present. Other patterns were detected with <100% specificity and these would require further testing by other means to definitively identify abnormal haemoglobins.

Conclusions: Although the ID-particle technique is a simple and cheap technique with high sensitivity, specificity and PPV compared with cellulose acetate electrophoresis in detecting HbSS, it could not differentiate HbAS from HbSS with high levels of HbF. High environmental temperatures could melt the test microtubes. Cellulose acetate electrophoresis remains the technique of choice for the screening of abnormal haemoglobins in the Sudan.

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