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Annals of Clinical Biochemistry

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This version was published on 1 March 2009
Ann Clin Biochem 2009;46:159-161
doi:10.1258/acb.2008.008212
© 2009 Association for Clinical Biochemistry

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Short Reports

Stability of chemical and immunochemical analytes in uncentrifuged plasma samples

Aila Leino1,2 and M K Koivula1


1 TYKSLAB, the Hospital District of Southwest Finland; 2 Department of Clinical Chemistry, University Hospital, Kiinamyllynkatu 4-8, FIN-20520, Turku, Finland


Corresponding author: Aila Leino. Email aila.leino{at}tyks.fi

Background: The stability of analyte concentrations in plasma after prolonged contact with blood cells in uncentrifuged lithium-heparin gel tubes was studied.

Methods: To investigate the stability of concentrations of 26 chemistry and 15 immunochemistry analytes, the simultaneously drawn samples (n = 50) were measured after 6 h storage at +8°C and +22°C in whole blood and after immediate separation of plasma. The analyte concentrations were measured with a Roche Modular PPEE analyser using reagents from Roche Diagnostics.

Results: After prolonged contact with cells a clinically significant change was only observed for potassium where the mean value increased from 4.0 mmol/L to 4.8 mmol/L (P < 0.001) when stored at +8°C.

Conclusion: Immediate separation of plasma from cells is recommended. However, when prolonged contact of plasma with cell is unavoidable, samples can be kept uncentrifuged for up to 6 h at +8°C or at +22°C. The stability of potassium, however, is temperature-dependent and cannot be measured from refrigerated blood samples.


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