RSM logo
Annals of Clinical Biochemistry

Home Current issue Browse archive Alerts About the journal Feedback
 
This version was published on 1 March 2009
Ann Clin Biochem 2009;46:159-161
doi:10.1258/acb.2008.008212
© 2009 Association for Clinical Biochemistry

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
acb.2008.008212v1
46/2/159    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Leino, A.
Right arrow Articles by Koivula, M K
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Short Reports

Stability of chemical and immunochemical analytes in uncentrifuged plasma samples

Aila Leino1,2 and M K Koivula1


1 TYKSLAB, the Hospital District of Southwest Finland; 2 Department of Clinical Chemistry, University Hospital, Kiinamyllynkatu 4-8, FIN-20520, Turku, Finland


Corresponding author: Aila Leino. Email aila.leino{at}tyks.fi

Background: The stability of analyte concentrations in plasma after prolonged contact with blood cells in uncentrifuged lithium-heparin gel tubes was studied.

Methods: To investigate the stability of concentrations of 26 chemistry and 15 immunochemistry analytes, the simultaneously drawn samples (n = 50) were measured after 6 h storage at +8°C and +22°C in whole blood and after immediate separation of plasma. The analyte concentrations were measured with a Roche Modular PPEE analyser using reagents from Roche Diagnostics.

Results: After prolonged contact with cells a clinically significant change was only observed for potassium where the mean value increased from 4.0 mmol/L to 4.8 mmol/L (P < 0.001) when stored at +8°C.

Conclusion: Immediate separation of plasma from cells is recommended. However, when prolonged contact of plasma with cell is unavoidable, samples can be kept uncentrifuged for up to 6 h at +8°C or at +22°C. The stability of potassium, however, is temperature-dependent and cannot be measured from refrigerated blood samples.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




Acute CT - A Primer of Emergency Imaging

 RSM Journals - Obstetric Medicine RSM Journals - Hand Therapy