Frontline ® immunochromatographic device for on-site urine testing of amphetamines: laboratory validation using authentic specimens

Ann Clin Biochem 2000;37:199-204
doi:10.1258/0004563001899005
© 2000 Association for Clinical Biochemistry

 

This Article
Right arrow

Full Text (PDF)

Right arrow
Alert me when this article is cited
Right arrow
Alert me if a correction is posted
Services
Right arrow
Email this article to a friend
Right arrow

Similar articles in this journal

Right arrow
Similar articles in PubMed
Right arrow
Alert me to new issues of the journal
Right arrow
Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow
Articles by Beck, O
Right arrow
Articles by Wennig, R
Right arrow Search for Related Content
PubMed
Right arrow
PubMed Citation
Social Bookmarking

What’s this?

Original Articles


O Beck,
M Kraft,
M R Moeller,
B L Smith,
S Schneider and
R Wennig

We evaluated a new test device for amphetamines and methamphetamines (Frontline ®, cut-off limit 300 ng/mL) using authentic clinicaland forensic specimens. The device is based on immunochromatographyand is dipped into urine and read visually by comparison witha colour scale after a few minutes. A total of 658 specimenswere tested by comparing results of the screening procedurewith established immunoassays. Discordant results were furtherinvestigated by gas chromatography- mass spectrometry or gaschromatography (with flame ionization detector). The Frontlinedevice had a sensitivity of 93% and a specificity of 98%. Whenspecimens were classified by urine amphetamine concentration,close agreement was obtained at concentrations below 150 ng/mLand above 1000 ng/mL. A small number of specimens with amphetamineconcentrations between 300 and 1000 ng/mL tested negative inthe Frontline test. This finding could to some extent be explainedby the enantioselectivity of the antibodies in the Frontlinetest to d-amphetamine. We conclude that the performance of theFrontline test device for amphetamines is adequate for presumptiveclinical and forensic screening.


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What’s this?