Analysis of urine for drugs of abuse using mixed-mode solid-phase extraction and gas chromatography-mass spectrometry

Ann Clin Biochem 2000;37:690-700
doi:10.1258/0004563001899744
© 2000 Association for Clinical Biochemistry

This Article
Right arrow

Full Text (PDF)

Right arrow
Alert me when this article is cited
Right arrow
Alert me if a correction is posted
Services
Right arrow
Email this article to a friend
Right arrow

Similar articles in this journal

Right arrow
Similar articles in PubMed
Right arrow
Alert me to new issues of the journal
Right arrow
Download to citation manager
Right arrow
Citing Articles
Right arrow
Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow
Articles by Paterson, S.
Right arrow
Articles by Houldsworth, P.
Right arrow Search for Related Content
PubMed
Right arrow
PubMed Citation
Social Bookmarking

What’s this?

Original Articles


Susan Paterson,
Rosa Cordero,
Scott McCulloch and
Philip Houldsworth

A method for the simultaneous analysis of urine for the major drugs of abuse is described. The analytical procedure uses solid-phase extraction (SPE), gas chromatography-mass spectrometry (GC-MS) and a semi-automated identification process. It allows simultaneous extraction, derivatization and analysis of acidic, neutral and basic drugs from urine. Urine samples were subjected to enzymatic hydrolysis followed by SPE using Bakerbond narc-2 columns. The eluant was selectively derivatized with N-methyl-bis-trifluoroacetamide (MBTFA) and N-methyl-N-trimethylsilyltrifluoroacetamide + 1%trimethylchlorosilane. Analysis was performed using a GC-MSsystem operating in full scan mode. A simple macro programmewas written to enhance the mass spectra identification capabilitiesof the MS software by producing extracted ion chromatograms(EIC) for the drugs of interest. Once a suspect compound wasindicated by EIC, the mass spectrum of the compound was searchedmanually against reference libraries for positive identificationand the retention time checked against that of the standard.This procedure has increased both the amount and the reliabilityof information given to clinicians without increasing the costper sample. The system has been in routine operation for 24months, processing up to 40 urine samples per day, with a usualturn-around time of 48 h.


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What’s this?






This article has been cited by other articles:

Home page J. Clin. Pathol.Home page

R Rajab, E Stearns, and S Baithun
Autopsy pathology of cocaine users from the Eastern district of London: a retrospective cohort study
J. Clin. Pathol.,

July 1, 2008;
61(7):
848 – 850.

[Abstract]
[Full Text]
[PDF]


Home page Br. J. PsychiatryHome page

T. WEAVER, P. MADDEN, V. CHARLES, G. STIMSON, A. RENTON, P. TYRER, T. BARNES, C. BENCH, H. MIDDLETON, N. WRIGHT, et al.
Comorbidity of substance misuse and mental illness in community mental health and substance misuse services
The British Journal of Psychiatry,

October 1, 2003;
183(4):
304 – 313.

[Abstract]
[Full Text]
[PDF]