Effect of storage of plasma and serum on enzymatic determination of non-esterified fatty acids

Ann Clin Biochem 2001;38:252-255
© 2001 Association for Clinical Biochemistry


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Original Articles

Luis García Menéndez,
Ana L Fernández,
Alfredo Enguix,
Constanza Ciriza and
Juan Amador

Many contradictory results have been published on the stabilityof total non-esterified fatty acids in blood, plasma and serumunder different storage conditions. The present study was undertakento investigate the stability of non-esterified fatty acids,measured with an enzymatic method, in samples of EDTA-treatedplasma and serum under different temperature conditions. Weconclude that EDTA-treated plasma and serum can both be usedfor analysis. Specific reference values should be establisheddepending on the type of sample chosen. Samples that cannotbe analysed immediately can be stored at -20°C for at least14 days without significant changes in the concentration oftotal non-esterified fatty acids. None of the other storageconditions and periods studied are suitable for the measurementof non-esterified fatty acid concentration.

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