Paracetamol-associated interference in an HPLC-ECD assay for urinary free metadrenalines and catecholamines

Ann Clin Biochem 2004;41:316-320
© 2004 Royal Society of Medicine

This Article
Right arrow

Full Text (PDF)

Right arrow
Alert me when this article is cited
Right arrow
Alert me if a correction is posted
Right arrow
Email this article to a friend
Right arrow

Similar articles in this journal

Right arrow
Similar articles in PubMed
Right arrow
Alert me to new issues of the journal
Right arrow
Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow
Articles by Davidson, F. D
Right arrow Search for Related Content
Right arrow
PubMed Citation
Social Bookmarking

What’s this?

Original Articles

Fraser D Davidson

Biochemistry Department, Crosshouse Hospital, Kilmarnock KA2 0BE, UK

Background: Some high-performance liquid chromatography techniqueswith electrochemical detection for urinary catecholamines andtheir metabolites can be subject to interference from drugsand their metabolites. Prime amongst these interferences arethose due to paracetamol ingestion.

Methods: The prevalence of paracetamol contamination was determinedby measuring the drug in all patient specimens submitted forcatecholamine analysis over a 2-month period. These findingswere then related to the proportion of unreportable resultsat each range of specimen paracetamol concentration. The apparentresults from a small representative sample of the paracetamol-positivespecimens are illustrated.

Results: Approximately one-third of urine specimens were foundto contain paracetamol. Low-level contamination can produceapparent patterns of results which may easily be confused withthose found in predominantly adrenaline-, or metadrenaline-secretingphaeochromocytomas.

Conclusion: Despite the fact that significantly less than 5%of an oral dose of paracetamol is excreted as the free drug,its analysis provides a good surrogate marker for the likelihoodof spurious results. It is useful to know prior to urinary freemetadrenaline or catecholamine analysis which urine specimensare likely to contain potentially interfering paracetamol metabolites.

CiteULike    Complore    Connotea    Digg    Reddit    Technorati    What’s this?