Analysis of hTERT expression in exfoliated cells from patients with bladder transitional cell carcinomas using SYBR green real-time fluorescence quantitative PCR

Ann Clin Biochem 2007;44:523-528
doi:10.1258/000456307782268093
© 2007 Association for Clinical Biochemistry

 

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Original Articles


Xiao-Yan Xie,
Xin Yang,
Jia-Hua Zhang and
Ze-Jun Liu


Department of Urology, Southwest Hospital, Third Military Medical University, Chongqing 400038, PRC;
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, PRC;
Department of Urology, Southwest Hospital, Third Military Medical University, Chongqing 400038, PRC;
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, PRC


Aims: To investigate whether hTERT mRNA expression could beused as a diagnostic and prognostic marker of bladder transitionalcell carcinoma.

Methods: SYBR green I real-time fluorescence quantitative PCR method was used to quantify hTERT mRNA expression in exfoliatedcells from patients with bladder transitional cell carcinomas.

Results: A significant correlation was observed between the level of hTERT mRNA expression in exfoliated cells collectedin urine with pathological grade and clinical stage.

Conclusions: Our results show that SYBR green real-time fluorescence quantitative evaluation of hTERT mRNA could be a useful markerof tumor progression for the early diagnosis and prognosis ofbladder cancers.


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