Antioxidant enzymes, glutathione and lipid peroxidation in peripheral blood of children affected by coeliac disease

Ann Clin Biochem 2007;44:537-543
doi:10.1258/000456307782268075
© 2007 Association for Clinical Biochemistry

 

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Original Articles


Vesna Stojiljkovi,
Ana Todorovi,
Nedeljko Radlovi,
Sneana Peji,
Marija Mladenovi,
Jelena Kasapovi and
Sneana B Pajovi


Laboratory of Molecular Biology and Endocrinology, Vina Institute of Nuclear Sciences, Belgrade, Serbia;
University Children’s Hospital, Belgrade, Serbia;
Health Centre, Valjevo, Serbia


Background: Oxidative stress has been implicated in the pathogenesisof coeliac disease. The aim of this study was to examine themodulation of the biochemical response to oxidative stress inuntreated and treated coeliac disease.

Methods: The study involved peripheral blood samples from 39paediatric patients (18 with active, 11 with silent form ofthe disease, 10 on gluten-free diet [GFD]) and 30 control subjects.The activities of superoxide dismutase (SOD), catalase (CAT),glutathione peroxidase (GPx) and glutathione reductase (GR),as well as the concentrations of total glutathione (GSH) andlipid hydroperoxides (LOOH) were determined in patients andcontrols.

Results: In comparison to the controls, a significant increase in SOD activity was found in the active group (P<0.05), while CAT activity was elevated in GFD group (P<0.05). GPx activity was lower in patients than in controls (active and silent, P<0.001; GFD, P<0.01). GSH contents were significantly reduced in all patient groups (P<0.001) as well, while the concentration of LOOH was elevated in active and silent group (P<0.001). The concentration of LOOH correlated negatively with the activity of GPx (r = -0.32, P<0.01) and the concentration of GSH (r = -0.70, P<0.001). A significant positive correlation was found between the concentration of GSH and the activity of GPx (r = 0.57, P<0.001).

Conclusions: The results show evidence of increased oxidativestress in untreated coeliac disease. Although LOOH were notsignificantly elevated in the GFD group, changes in antioxidantenzyme activities and GSH content demonstrate that oxidativestress persists even in treated patients.

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