Evaluation of a method for measuring tissue non-specific alkaline phosphatase activity in healthy subjects

Ann Clin Biochem 2007;44:544-548
doi:10.1258/000456307782268165
© 2007 Association for Clinical Biochemistry

 

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Original Articles


Makoto Matsushita,
Tsutomu Irino,
Kiyoshi Kamiyama,
Yoshimi Muramoto,
Takeshi Kawaguchi,
Takanari Nakano and
Tsugikazu Komoda


Department of Clinical Chemistry, Saitama Prefectural University, 820 Sannomiya, Koshigaya 343-8540, Japan;
Department of Clinical Chemistry, Saitama Prefectural University, 820 Sannomiya, Koshigaya 343-8540, Japan;
Department of Clinical Laboratory, Medical Diagnostic Center of Urawa Medical Association, Saitama, Japan;
Department of Central Clinical Laboratory, Cardiovascular Institute, Tokyo, Japan;
Department of Biochemistry, Faculty of Medicine, Saitama Medical University, Saitama, Japan;
Department of Biochemistry, Faculty of Medicine, Saitama Medical University, Saitama, Japan;
Department of Biochemistry, Faculty of Medicine, Saitama Medical University, Saitama, Japan


Background: Intestinal alkaline phosphatase (IAP) isozymes inthe healthy human serum samples appears in two isoforms: normal-molecular-weightIAP (NIAP) and high-molecular-weight IAP (HIAP). We have demonstratedthat the reference range for serum alkaline phosphatase (ALP)activity is higher in blood group B and O antigen secretorsthan in the tested other blood groups, for the appearance ofthese isoforms is depended on blood group B or O antigen secretors.

Methods: We assessed a diethanolamine-L-phenylalanine (DEA-Phe) method for measuring tissue non-specific alkaline phosphatase (TNAP). This assays the sum of liver alkaline phosphatase and bone alkaline phosphatase activities as determined by an inhibiting IAP activity method with Phe. We classified 420 healthy subjects into two groups, a group of subjects who had blood group B or O antigen secretors (n = 184) and a group of subjects who had other blood groups (n = 236).

Results: ALP activity was higher in the B or O secretor group than in the other group: 20.9% higher (P<0.001) by the N-methyl-D-glucamine method, 13.7% higher (P<0.002) by 2-amino-2-methyl-1-propanol method, and 9.6% higher (P<0.05) by the diethanolamine method,but there was no significant difference in TNAP activity betweenthe two blood group when measured by the DEA-Phe method.

Conclusions: These results of this study support the expectationthat the DEA-Phe method would be specific for TNAP activity.In addition, the reference range for TNAP activity did not varywith the differences in the tested all blood groups.


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