Automated assay for plasma D-lactate by enzymatic spectrophotometric analysis with sample blank correction

Ann Clin Biochem 2008;45:177-183
doi:10.1258/acb.2007.007088
© 2008 Association for Clinical Biochemistry

 

This Article
Right arrow
Figures Only
Right arrow
Full Text
Right arrow

Full Text (PDF)

Right arrow
Alert me when this article is cited
Right arrow
Alert me if a correction is posted
Services
Right arrow
Email this article to a friend
Right arrow

Similar articles in this journal

Right arrow
Similar articles in PubMed
Right arrow
Alert me to new issues of the journal
Right arrow
Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow
Articles by Herrera, D. J.
Right arrow
Articles by Griffiths, P.
Right arrow Search for Related Content
PubMed
Right arrow
PubMed Citation
Social Bookmarking

What’s this?

Original Article


Daniel Juan Herrera 1 ,
Kevin Morris 2,
Chris Johnston 1 and
Paul Griffiths 1


1 Department of Clinical Chemistry;
2 Intensive Care Unit, Birmingham Children’s Hospital, Steelhouse Lane, Birmingham B4 6NH, UK


Corresponding author: Daniel Juan Herrera. Email: daniel.herrera{at}bch.nhs.uk


Background: D-lactate is essentially a product of bacterial metabolism,and its assessment in plasma has been mainly used to diagnoseD-lactic acidosis in patients with short bowel syndrome. Inthe last few years, there has been growing interest in the useof subclinical elevations of D-lactate concentrations as a diagnostictool in a variety of clinical conditions such as ischaemia,trauma or infection.

Methods: An endpoint enzymatic spectrophotometric assay to measure plasmaD-lactate with a sample blank correction was validated on ourroutine clinical chemistry analyser (Olympus AU640). An ultrafiltrationprocedure was used in samples with a high L-lactate dehydrogenase(L-LDH) activity in order to avoid underestimation of the D-lactateconcentration, when a sample blank was processed.

Results: The intra- and inter-assay imprecision were <5.1% and <13.3%, respectively and the mean recovery for the D-lactate assay was 95% (range 88–103%). Samples with L-LDH activity greater than 1500 IU/L required the use of ultrafiltration devices. Plasma D-lactate concentration in our ‘non-diseased’ paediatric population showed a non-Gaussian distribution – 95th percentile equal to 19 µmol/L – and no differencebased on gender or age was observed.

Conclusion: We have established an accurate, sensitive and precise routineassay for D-lactate measurement in plasma. The assay was usedto formulate paediatric reference ranges and will be used toassist clinicians to evaluate ‘D-lactate toxicity’in patients with a variety of conditions such as short bowelsyndrome, small bowel transplantation and as an early markerof intestinal ischaemia.


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What’s this?