Hyperhomocysteinaemia and parameters of antioxidative defence in Tunisian patients with coronary heart disease

Ann Clin Biochem 2008;45:193-198
© 2008 Association for Clinical Biochemistry


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Original Article

Mohsen Kerkeni 1 ,
Faouzi Added 2,
Mohamed Ben Farhat 2,
Abdelhedi Miled 3,
François Trivin 4 and
Khira Maaroufi 1

1 Research Unit 03/UR/08-14, Faculty of Pharmacy, 5000 Monastir, Tunisia;
2 Department of Cardiology, CHU Fattouma Bourguiba, Monastir, Tunisia;
3 Department of Biochemistry and Toxicology, CHU Hached, Sousse, Tunisia;
4 Department of Clinical Biochemistry, Francois Rabelais University, Tours, France

Corresponding author: Dr Mohsen Kerkeni, Research Unit 03/UR/08-14, Faculty of Pharmacy, 5000 Monastir. Email: Mohsen.kerkeni{at}yahoo.fr

Background: An imbalance between oxidative damage and antioxidative protectionin association with the pathophysiology of atherosclerosis hasbeen suggested. The aim of this study was to test the parametersof antioxidative defence and to assess their association withhyperhomocysteinaemia and the severity of coronary heart disease(CHD) in Tunisian patients.

Methods: The study population included 100 patients with CHD and 120healthy controls. The severity of CHD was expressed as the numberof affected vessels. Superoxide dismutase (SOD) activity, glutathioneperoxidase (GPx) activity and total antioxidant status (TAS)concentrations were measured using commercially available methods.Plasma total homocysteine (tHcy) concentration was determinedby direct chemiluminescence assay. Serum zinc (Zn) was measuredby a colorimetric method.

Results: Compared with healthy control subjects, patients with CHD had significantly lower activities of SOD (P < 0.01), GPx (P < 0.001), and serum Zn concentrations (P < 0.001) and significantly higher tHcy concentration (P < 0.001). However TAS concentrations were not significantly different between the groups. SOD and GPx activities were negatively correlated with tHcy concentration (P < 0.05, P < 0.001, respectively).Patients with hyperhomocysteinaemia showed a lower GPx and SODactivities than patients with normohomocysteinaemia. Antioxidantenzyme activities tended to be decreased in CHD patients presentingwith 0- to 3-vessel stenosis.

Conclusions: This study indicates that low activity of GPx, SOD and Zn concentrationare associated with CHD patients. We hypothesize that hyperhomocysteinaemiaand low antioxidant enzyme activities may increase the extentof CHD.

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