Two-year stability of NT-proBNP in frozen samples using the Roche Elecsys system

Ann Clin Biochem 2008;45:318-319
© 2008 Association for Clinical Biochemistry


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Short Reports

B Cauliez,
J Guignery,
S Marinier,
I Mariau and
A Lavoinne

Laboratoire de Biochimie Médicale, CHU de Rouen-Hôpital Charles Nicolle, 76031 Rouen-Cedex, France

Corresponding author: Dr Bruno Cauliez. Email: Bruno.Cauliez{at}

Background: The in vitro stability of N-terminal (NT)-proBNP (brain natriureticpeptide) at room temperature and at 4°C is excellent andhas been well studied. However, less is known concerning itsstability after a long-term frozen storage. This notion couldbe of major interest in the context of clinical evaluations.

Methods: NT-proBNP was measured on 97 heparinized samples before andafter a two-year frozen storage (–20°C) using theRoche Elecsys system.

Results: There is a slight but significant decrease of NT-proBNP concentration after frozen storage. However, this decrease is <10% for more than 90% of the samples and the maximum decrease is 16%. Moreover, values on frozen samples are well correlated with values on fresh samples (r = 0.99) using the following equation: NT-proBNPstored sample = 0.93 NT-proBNPfresh sample + 81. A similar equation was found in lower concentrations (NT-proBNP ≤ 2000 ng/L).

Conclusions: These data show that NT-proBNP can be stored for at least twoyears at –20°C before measurement without a substantialloss of immunoreactivity.

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