Measurement of plasma lysophosphatidic acid concentration in healthy subjects: strong correlation with lysophospholipase D activity

Ann Clin Biochem 2008;45:364-368
© 2008 Association for Clinical Biochemistry

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Original Articles

Shigemi Hosogaya1,
Yutaka Yatomi2,3,
Kazuhiro Nakamura2,
Ryunosuke Ohkawa2,
Shigeo Okubo2,
Hiromitsu Yokota2,
Masato Ohta1,
Hirokazu Yamazaki1,
Toru Koike1 and
Yukio Ozaki1

1 Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Yamanashi;
2 Department of Clinical Laboratory, The University of Tokyo Hospital;
3 Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan

Corresponding author: Dr Yutaka Yatomi. Email: yatoyuta-tky{at}

Background: Lysophosphatidic acid (LPA) plays important roles in a varietyof biological responses, especially in the area of vascularbiology, and the determination of its plasma concentration isbelieved to be important. Several mechanisms are known to beinvolved in the metabolism of LPA.

Methods: To identify factors that may determine the plasma concentrationsof this important bioactive lipid, we examined its concentrationsusing an enzymatic cycling assay and related parameters in 146healthy subjects.

Results: The LPA concentration was significantly higher in women (mean ± SD, 0.103 ± 0.032 µmol/L; n = 47) than in men (0.077 ± 0.026 µmol/L; n = 99). A multipleregression analysis showed a strong positive correlation betweenthe plasma LPA concentration and serum lysophospholipase D (lysoPLD)activity, while the LPA concentration was correlated with theplasma lysophosphatidylcholine (LPC) concentration only in men.Other lipid-related parameters were only slightly correlatedor were not correlated with the LPA concentration.

Conclusions: Our findings suggested that conversion from LPC by lysoPLD mightbe the major route for LPA production in plasma.

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