Homocysteine enhances LDL fatty acid peroxidation, promoting microalbuminuria in type 2 diabetes

Ann Clin Biochem 2008;45:476-480
© 2008 Association for Clinical Biochemistry


This Article
Right arrow
Full Text
Right arrow

Full Text (PDF)

Right arrow
Alert me when this article is cited
Right arrow
Alert me if a correction is posted
Right arrow
Email this article to a friend
Right arrow

Similar articles in this journal

Right arrow
Similar articles in PubMed
Right arrow
Alert me to new issues of the journal
Right arrow
Download to citation manager
Right arrow
Citing Articles
Right arrow

Citing Articles via Web of Science (1)

Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow
Articles by Kassab, A.
Right arrow
Articles by Hammami, M.
Right arrow Search for Related Content
Right arrow
PubMed Citation
Social Bookmarking

What’s this?

Original Articles

Asma Kassab1,
Thouraya Ajmi2,
Manel Issaoui3,
Larbi Chaeib4,
Abdelhedi Miled1 and
Mohamed Hammami3

1 Biochemistry Laboratory, CHU F Hached Sousse;
2 Epidemiology Unit, Medicine Faculty Sousse;
3 Biochemistry Department, Medicine Faculty Monastir;
4 Endocrinology Unit, CHU F Hached Sousse, Tunisia

Corresponding author: Dr Asma Kassab. Email: kassab.asma{at}laposte.net

Background: We aimed to establish the relationship between glycated haemoglobin (HbA1c), hypertension and microalbuminuria onset in type 2 diabetes.We also intended to ascertain the metabolic action of homocysteineon LDL fatty acids and on renal function.

Methods: The study was carried out on 200 patients with type 2 diabetes and 200 healthy subjects. HbA1c, apolipoprotein B (apo B) andmicroalbuminuria were measured using immunoturbidimetric methods.Cholesterol, peroxide, urea and uric acid were assayed usingcolorimetric methods. Creatinine clearance was calculated usingthe Cockroft-Gault equation. Homocysteine was measured by immunologicalfluorescence polarization. LDL fatty acids were quantified bygas chromatography.

Results: Creatinine and microalbuminuria significantly increased in type 2 diabetes when compared with controls. Microalbuminuria was significantly correlated with HbA1c and with the presence of high blood pressure. Homocysteinaemia significantly correlated with creatinine clearance in diabetes. Linoleic acid (C18:2{omega}6) did not differ between groups. C18:2{omega}6/C18:3{omega}3 ratio was three times higher in diabetics than in controls. Total saturated fatty acids, homocysteine, H2O2 and LDL-thiobarbituric reactive substances significantly increased in microalbuminuric when compared with normoalbuminuric diabetes. Total polyunsaturated fatty acids, arachidonic acid (C20:4{omega}6), LDL-cholesterol, apoB and creatinine clearance significantly decreased in microalbuminuricwhen compared with normoalbuminuric diabetes.

Conclusion: Microalbuminuria onset is associated with renal protein oxidation that is preceded by LDL fatty acid oxidation. The latter is initiated by H2O2 produced from an auto-oxidation of homocysteineand increased metabolism of arachidonic acid towards its pro-inflammatoryeicosanoids. An oxidative stress state is the common groundof diffused vasculopathy.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What’s this?