Platelet contamination causes large variation as well as overestimation of mitochondrial DNA content of peripheral blood mononuclear cells

Ann Clin Biochem 2008;45:513-514
doi:10.1258/acb.2008.008008
© 2008 Association for Clinical Biochemistry

 

 

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Short Reports


Michiyo Urata,
Yui Koga-Wada,
Yuzo Kayamori and
Dongchon Kang


Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, 3-1-1 Maidashi Higashi-ku, Fukuoka 812-8582, Japan


Corresponding author: Dr Kang. Email: kang{at}mailserver.med.kyushu-u.ac.jp


Background: Alterations in the copy number of mitochondrial DNA (mtDNA)play a role in the pathogenesis of mitochondrial diseases andother many common diseases. Recently, the copy number of leukocytemtDNA has been considered to serve as a biomarker to monitoror chase such diseases. Therefore, reproducible mtDNA measurementis required.

Methods: Peripheral blood mononuclear cells were prepared by a density-basedmethod. The mtDNA/cell was measured by quantitative realtimepolymerase chain reaction.

Results: The degree of platelet contamination varied to a large extentamong preparations. The mtDNA copy numbers per mononuclear cellwere 269 ± 51 and 146 ± 14 in the samples beforeand after the platelet depletion, respectively.

Conclusion: A density-based mononuclear cell preparation causes heavy plateletcontamination. The platelet depletion from a sample is particularlyimportant for comparing the mtDNA contents between differentdates or between different patients.


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