Platelet contamination causes large variation as well as overestimation of mitochondrial DNA content of peripheral blood mononuclear cells

Ann Clin Biochem 2008;45:513-514
© 2008 Association for Clinical Biochemistry



This Article
Right arrow
Full Text
Right arrow

Full Text (PDF)

Right arrow
Alert me when this article is cited
Right arrow
Alert me if a correction is posted
Right arrow
Email this article to a friend
Right arrow

Similar articles in this journal

Right arrow
Similar articles in PubMed
Right arrow
Alert me to new issues of the journal
Right arrow
Download to citation manager
Right arrow
Citing Articles
Right arrow

Citing Articles via Web of Science (1)

Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow
Articles by Urata, M.
Right arrow
Articles by Kang, D.
Right arrow Search for Related Content
Right arrow
PubMed Citation
Social Bookmarking

What’s this?

Short Reports

Michiyo Urata,
Yui Koga-Wada,
Yuzo Kayamori and
Dongchon Kang

Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, 3-1-1 Maidashi Higashi-ku, Fukuoka 812-8582, Japan

Corresponding author: Dr Kang. Email: kang{at}

Background: Alterations in the copy number of mitochondrial DNA (mtDNA)play a role in the pathogenesis of mitochondrial diseases andother many common diseases. Recently, the copy number of leukocytemtDNA has been considered to serve as a biomarker to monitoror chase such diseases. Therefore, reproducible mtDNA measurementis required.

Methods: Peripheral blood mononuclear cells were prepared by a density-basedmethod. The mtDNA/cell was measured by quantitative realtimepolymerase chain reaction.

Results: The degree of platelet contamination varied to a large extentamong preparations. The mtDNA copy numbers per mononuclear cellwere 269 ± 51 and 146 ± 14 in the samples beforeand after the platelet depletion, respectively.

Conclusion: A density-based mononuclear cell preparation causes heavy plateletcontamination. The platelet depletion from a sample is particularlyimportant for comparing the mtDNA contents between differentdates or between different patients.

CiteULike    Complore    Connotea    Digg    Reddit    Technorati    What’s this?