Measurement of cholesterol concentrations of major serum lipoprotein classes in haemodialysis patients by anion-exchange chromatography

This version was published on 1 November 2008

Ann Clin Biochem 2008;45:571-574
doi:10.1258/acb.2008.008018
© 2008 Association for Clinical Biochemistry

 

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Original Articles


Yuji Hirowatari1,
Hiroshi Yoshida2,
Yuriko Fueki3,
Masayuki Ito3,
Yutaka Ogura1,
Nobuyuki Sakurai4 and
Takashi Miida5


1 Bioscience Division, Tosoh Corporation, Kanagawa, Japan;
2 Department of Laboratory Medicine, Jikei University Kashiwa Hospital, Chiba, Japan;
3 Graduate School of Medical and Dental Sciences Medicine, Niigata University, Niigata, Japan;
4 Murakami Memorial Hospital, Niigata, Japan;
5 Department of Clinical Laboratory Medicine, Juntendo University Hospital, Tokyo, Japan


Corresponding author: Dr Yuji Hirowatari. Email: hirowata{at}tosoh.co.jp


Background: Increased triglyceride (TG)-rich lipoproteins and decreasedHDL that are implicated in the progression of atheroscleroticvascular diseases, are present in serum samples of patientsundergoing haemodialysis (HD) therapy. Therefore, it is importantto measure serum TG-rich lipoprotein concentrations to preventthe diseases.

Methods: The cholesterol concentrations of lipoprotein classes in serum samples from the HD patients (n = 18) and healthy subjects (n= 18) were analysed by our recently developed method of high-performanceliquid chromatography (HPLC), in which the lipoprotein classeswere separated using an anion-exchange column, and the cholesterolconcentrations of each of those were measured enzymaticallyusing a post-column reaction. The ability of fractionated lipoproteincholesterol determination by this HPLC method is mostly equivalentto the determination ability of an ultracentrifugation (UC).

Results: HDL, LDL, and TG-rich lipoproteins, i.e. IDL, VLDL and chylomicrons, were well separated in the chromatograms. HDL cholesterol concentrations in the HD patients were significantly lower than in the healthy subjects (P < 0.0001), and IDL cholesterol concentrations and VLDL cholesterol concentrations in the HD patients were significantly higher than in the healthy subjects (P < 0.05).Profiles of these measured lipoprotein values were consistentwith the previously reported lipoprotein values, measured ultracentrifugallycharacteristic of HD patients.

Conclusion: These results suggest that the HPLC method may be sufficientlyapplied to the assessment of serum lipoprotein profile in HDpatients in place of the other method including an UC.


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