Standardization of the IMMULITE systems growth hormone assay with the recombinant IS 98/574

This version was published on 1 November 2008

Ann Clin Biochem 2008;45:598-600
doi:10.1258/acb.2008.008074
© 2008 Association for Clinical Biochemistry

 

This Article
Right arrow
Figures Only
Right arrow
Full Text
Right arrow

Full Text (PDF)

Right arrow
All Versions of this Article:

acb.2008.008074v1

45/6/598

most recent

Right arrow
Alert me when this article is cited
Right arrow
Alert me if a correction is posted
Services
Right arrow
Email this article to a friend
Right arrow

Similar articles in this journal

Right arrow
Similar articles in PubMed
Right arrow
Alert me to new issues of the journal
Right arrow
Download to citation manager
Right arrow
Google Scholar
Right arrow
Articles by Barth, J. H
Right arrow
Articles by Sibley, P. E C
PubMed
Right arrow
PubMed Citation
Social Bookmarking

What’s this?

Short Reports


Julian H Barth1 and
Paul E C Sibley2


1 Department of Clinical Biochemistry and Immunology, Leeds General Infirmary, Leeds LS1 3EX;
2 Siemens Healthcare Diagnostics, Glyn Rhonwy, Llanberis LL55 4EL, UK


Corresponding author: Dr Julian H Barth. Email: julian.barth{at}leedsth.nhs.uk


Background: The diagnosis of disorders of growth hormone (GH) secretionis based on the measurement of GH before and after dynamic stimulationor suppression tests. Over the years, specific cut-off valueshave been proposed without taking account of the considerablevariation in results obtained using different methods for analysingGH. Recent publications have recommended the standardizationof GH assays using IS 98/574, which is calibrated in mass andunits (U). The IMMULITE range of GH assays have been recalibratedusing this standard.

Methods: We analysed 745 samples using the current and the restandardizedGH assay kits. This series of samples contained 90 stimulationtests and seven suppression tests.

Results: The data comparing the current and restandardized assays show a similar relationship for both basal and stimulated GH values for all IMMULITE platforms. There is a negative bias by the restandardized assay above approximately 20 µg/L. A conversioncan be derived from this study which would mean that patientGH results expressed in milliunits per litre of IMMULITE in-housederived standard will be approximately three-fold of that expressedin micrograms per litre of IS 98/574.

Conclusion: Use of IS 98/574 offers standardized reporting in microgramper litre IS 98/574. This will permit compliance with the consensusrecommendations and the development of a firm evidence basefor defining decision-making GH values.


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What’s this?