Differentially displayed proteins as a tool for the development of type 2 diabetes

This version was published on 1 July 2009

Ann Clin Biochem 2009;46:306-310
© 2009 Association for Clinical Biochemistry


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Original Articles

Ayse Dincer1,
Secil Onal1,
Suna Timur1,
Ali Zeytunluoglu1,
Erdal Duman2 and
Figen Zihnioglu1

1 Faculty of Science, Biochemistry Department, Ege University, Bornova-Izmir;
2 Izmir Atatürk State Hospital, Endocrinology Division, Izmir, Turkey

Corresponding author: Figen Zihnioglu, Biochemistry Department, Ege University, Faculty of Science, 35100 Bornova Izmir, Turkiye. Email: figen.zihnioglu{at}ege.edu.tr

Background: Type 2 diabetes is a complex disease that still requires a greatdeal of work to be carried out to understand the pathophysiology.Recently, researchers have focused on studying the organs andtissues known to be involved in the development of the type2 phenotype using a proteomic approach. Little work has beenreported on plasma of type 2 diabetics in whom the clinicalstatus has been well characterized. In this study, changes inplasma proteins of type 2 diabetics were investigated by proteomicanalysis in well-characterized individuals with type 2 diabetes(early and late stage) and control groups (with or without afamily history of diabetes).

Methods: Samples were analysed by two-dimensional gel electrophoresisand significantly differentiated proteins were identified bynano-LC-ESI-MS.

Results: A total of 12 protein signatures that were differentially displayedwith high significance compared with controls were selected.Four of the differentially displayed proteins were identifiedas haptoglobin alpha2, haptoglobin Hp2(fragment) and transthyretinand Chain A (formerly prealbumin), and all were up-regulated.Thiol-specific antioxidant protein, Chain A, tertiary structuresof three amyloidogenic transthretin variants and haptoglobin-relatedprotein precursor were all down-regulated in controls with afamily history of diabetes, early and late diabetic patientsin comparison with the control.

Conclusion: A proteomic-based approach was used to discover and identifythe differentially expressed proteins in various states of type2 diabetes.

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