Evaluation of redox statuses in patients with hepatitis B virus-associated hepatocellular carcinoma

This version was published on 1 September 2009

Ann Clin Biochem 2009;46:394-400
doi:10.1258/acb.2009.009029
© 2009 Association for Clinical Biochemistry

 

This Article

Figures Only

Full Text

Full Text (PDF)


All Versions of this Article:

acb.2009.009029v1

acb.2009.009029v2

46/5/394

most recent


Alert me when this article is cited

Alert me if a correction is posted
Services

Email this article to a friend

Similar articles in this journal


Similar articles in PubMed

Alert me to new issues of the journal

Download to citation manager

Google Scholar

Articles by Tsai, S.-M.

Articles by Tsai, L.-Y.
PubMed

PubMed Citation
Social Bookmarking

What’s this?

Original Articles


Shih-Meng Tsai1,
Shu-Kai Lin2,
King-The Lee3,
Jen-Kuei Hsiao2,
Jung-Chih Huang2,
Szu-Hsien Wu4,5,
Hsu Ma4,5,
Szu-Hwa Wu2 and
Li-Yu Tsai2


1 Faculty of Medicine, College of Medicine;
2 Department of Medical Laboratory Science and Biotechnology, College of Health Sciences;
3 Department of Surgery, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung;
4 Division of Plastic Surgery, Department of Surgery, Taipei Veterans General Hospital;
5 Department of Surgery, Faculty of Medicine, College of Medicine, National Yang-Ming University, Taipei, Taiwan


Corresponding author: Li-Yu Tsai PhD, Department of Medical Laboratory Science and Biotechnology, College of Health Sciences, Kaohsiung Medical University, No. 100 Shi-Chuan 1st Road, Kaohsiung 80702, Taiwan. Email: tsliyu{at}kmu.edu.tw


Background: Excess reactive oxygen species related to neoplasia of liver has been established. Essentially, the human body has developed different antioxidant systems for defence against these attacks. To evaluate the redox status in hepatocellular carcinoma (HCC) induced by hepatitis B virus (HBV), the most important aetiological factor in Taiwan, changes in O2. generation, lipid peroxidationas well as antioxidant status in the blood of HCC patients withHBV carriers for more than 20 years were measured.

Methods: Superoxide anion radical (O2.–) generation and the levelsof malondialdehyde (MDA) served as an index of lipid peroxidationalong with the analyses of activities of superoxide dismutase(SOD), glutathione peroxidase (GPx) and glutathione reductase(GRx); also, glutathione status, including reduced glutathione(GSH) and oxidized glutathione (GSSG), and the levels of vitaminsA, C and E were determined.

Results: In 54 patients, the levels of O2.–, MDA and GSSG, andthe activities of SOD and GRx of blood were significantly higherthan those of 57 controls. Conversely, the levels of GSH andtotal GSH, and GSH/GSSG ratio, and vitamins A and C were significantlydecreased. Additionally, there were no significant changes inthe activity of GPx and the levels of vitamin E.

Conclusions: Our data suggest that the redox statuses in patients with HBV-associatedHCC were elevated or decreased in certain parameters. However,the increased activities of antioxidant enzymes may be a compensatoryup-regulation and the decrease antioxidant statuses were responsesto the enhanced oxidative stress in those patients.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What’s this?