Investigation of the potential association of vitamin D binding protein with lipoproteins

This version was published on 1 March 2010

Ann Clin Biochem 2010;47:143-150
doi:10.1258/acb.2009.009018
© 2010 Association for Clinical Biochemistry

 

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Original Articles


Marijn M Speeckaert1,
Youri E Taes2,
Marc L De Buyzere1,
Armand B Christophe2,
Jean-Marc Kaufman2 and
Joris R Delanghe1


1 Department of Clinical Chemistry, Ghent University Hospital;
2 Department of Endocrinology and Metabolic Diseases, Ghent University Hospital, Gent, Belgium


Corresponding author: Prof Dr Joris Delanghe, Department of Clinical Chemistry, Ghent University Hospital 2P8, De Pintelaan 185, 9000 Gent, Belgium. Email: joris.delanghe{at}ugent.be


Background: Vitamin D binding protein (DBP) acts as a vitamin D carrierand an actin scavenger. We have previously reported a correlationbetween serum DBP and lipid parameters in a cystic fibrosispopulation. In the present study, the relationship between serumDBP, lipoprotein fractions and vitamin D is investigated.

Methods: The presence of DBP in lipoprotein fractions was examined using precipitation, gel permeation chromatography and ultracentrifugation. The association between DBP and lipids was investigated in a cohort study of 211 men. Total and actin-free DBP concentrations were assessed by immunonephelometry and enzyme-linked immunosorbent assay. Serum 25(OH)- and 1.25(OH)2-vitamin D3 were assayed byradioimmunoassay. Total cholesterol, high-density lipoproteincholesterol (HDL), triglycerides and insulin were measured usingroutine methods. Low-density lipoprotein-cholesterol (LDL) wascalculated according to Friedewald’s formula.

Results: DBP was found to be present in very-low-density lipoprotein (VLDL). Gel permeation chromatography revealed a bimodal DBP distribution with a lipid-bound fraction besides the known free fraction. Ultracentrifugation confirmed the presence of DBP and 25(OH)-vitamin D3 in the VLDL particle. Total serum DBP concentration and the actin-bound DBP/DBP ratio correlated significantly with total cholesterol, LDL-cholesterol, triglycerides and albumin. The 25(OH)-vitamin D3/DBP ratio correlated negatively with serumtriglyceride concentration and body mass index (BMI). The actin-boundDBP complex was identified with Western blot.

Conclusions: The lipid-bound DBP fraction may be of greater importance thaninitially thought. The present findings may have clinical consequencesin view of the important physiological role of DBP.


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