Investigation of the potential association of vitamin D binding protein with lipoproteins

This version was published on 1 March 2010

Ann Clin Biochem 2010;47:143-150
© 2010 Association for Clinical Biochemistry


This Article
Right arrow
Figures Only
Right arrow
Full Text
Right arrow

Full Text (PDF)

Right arrow
All Versions of this Article:



most recent

Right arrow
Alert me when this article is cited
Right arrow
Alert me if a correction is posted
Right arrow
Email this article to a friend
Right arrow

Similar articles in this journal

Right arrow
Similar articles in PubMed
Right arrow
Alert me to new issues of the journal
Right arrow
Download to citation manager
Right arrow
Google Scholar
Right arrow
Articles by Speeckaert, M. M
Right arrow
Articles by Delanghe, J. R
Right arrow
PubMed Citation
Social Bookmarking

What’s this?

Original Articles

Marijn M Speeckaert1,
Youri E Taes2,
Marc L De Buyzere1,
Armand B Christophe2,
Jean-Marc Kaufman2 and
Joris R Delanghe1

1 Department of Clinical Chemistry, Ghent University Hospital;
2 Department of Endocrinology and Metabolic Diseases, Ghent University Hospital, Gent, Belgium

Corresponding author: Prof Dr Joris Delanghe, Department of Clinical Chemistry, Ghent University Hospital 2P8, De Pintelaan 185, 9000 Gent, Belgium. Email: joris.delanghe{at}

Background: Vitamin D binding protein (DBP) acts as a vitamin D carrierand an actin scavenger. We have previously reported a correlationbetween serum DBP and lipid parameters in a cystic fibrosispopulation. In the present study, the relationship between serumDBP, lipoprotein fractions and vitamin D is investigated.

Methods: The presence of DBP in lipoprotein fractions was examined using precipitation, gel permeation chromatography and ultracentrifugation. The association between DBP and lipids was investigated in a cohort study of 211 men. Total and actin-free DBP concentrations were assessed by immunonephelometry and enzyme-linked immunosorbent assay. Serum 25(OH)- and 1.25(OH)2-vitamin D3 were assayed byradioimmunoassay. Total cholesterol, high-density lipoproteincholesterol (HDL), triglycerides and insulin were measured usingroutine methods. Low-density lipoprotein-cholesterol (LDL) wascalculated according to Friedewald’s formula.

Results: DBP was found to be present in very-low-density lipoprotein (VLDL). Gel permeation chromatography revealed a bimodal DBP distribution with a lipid-bound fraction besides the known free fraction. Ultracentrifugation confirmed the presence of DBP and 25(OH)-vitamin D3 in the VLDL particle. Total serum DBP concentration and the actin-bound DBP/DBP ratio correlated significantly with total cholesterol, LDL-cholesterol, triglycerides and albumin. The 25(OH)-vitamin D3/DBP ratio correlated negatively with serumtriglyceride concentration and body mass index (BMI). The actin-boundDBP complex was identified with Western blot.

Conclusions: The lipid-bound DBP fraction may be of greater importance thaninitially thought. The present findings may have clinical consequencesin view of the important physiological role of DBP.

CiteULike    Complore    Connotea    Digg    Reddit    Technorati    What’s this?